Wright Lab, Duke University
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Methods

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The documents below describe protocols used in the Wright Lab.  For soil methodology, we draw heavily from the following book:

Robertson GP, Coleman DC, Bledoe CS, Sollins P, editors. 1999 Standard Soil Methods for Long-Term Ecological Research. Oxford, U.K.: Oxford University Press. pp. 272–288. ISBN 0195120833


• Soil sampling and sieving

• Soil moisture and organic matter

• Soil inorganic nitrogen (KCl) extraction and microbial biomass nitrogen extraction

• Soil Denitrification Enzymatic Assay (DEA)

• Soil Carbon mineralization

• Soil pH measurement

• Soil bulk density

•Determination of major secondary chemistry classes in invasive and native herbs

• Separate, weigh, and subsample vegetation for C:N analysis

•Weigh ground up plant material into tin capsules

• Atomic Absorption/Emission Spectrometer

•Flash EA CHN Analyzer





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